PKLR Antibody [Biotin] Summary
| Description |
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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| Immunogen |
Pyruvate Kinase from rabbit muscle
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| Localization |
Cytoplasmic
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| Specificity |
Cross reacts with all forms of muscle pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined.
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
PKLR
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| Purity |
Delipidation and Defibrination
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This product has been assayed against 1.0 ug of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:3,000 – 1:15,000 of the reconstitution concentration is suggested for this product.
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| Readout System |
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Reactivity Notes
Cross-reacts with Rabbit. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) – Immunoglobulin and Protease free
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| Preservative |
0.01% Sodium Azide
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| Purity |
Delipidation and Defibrination
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Alternate Names for PKLR Antibody [Biotin]
- EC 2.7.1.40
- PK1
- PK1PKRL
- PKL
- PKLR
- PKR
- Pyruvate Kinase 1
- pyruvate kinase isozyme R/L
- pyruvate kinase isozymes R/L
- pyruvate kinase type L
- pyruvate kinase, liver and blood cell
- pyruvate kinase, liver and RBC
- Red cell/liver pyruvate kinase
- RPK
- R-type/L-type pyruvate kinase
Background
Pyruvate kinase is also known as ATP:pyruvate phosphotransferase. At least three molecular forms with pyruvate kinase activity are known. PK1, the red cell form, is deficient in a type of hemolytic anemia. PK2 is found in kidney. PK3 is found in leukocytes, muscle, platelets, and brain but not in red cells or kidney. PK1 is found also in liver. During fetal development, PK3 changes to PK1 in the liver. PK1 is a tetramer composed of two dissimilar polypeptides of somewhat different molecular weight. It is an allosteric enzyme exhibiting cooperative binding for phosphoenolpyruvate and sensitivity to fructose-1,6-diphosphate. PK3 also is a tetrameric protein but, unlike PK1, all subunits are alike and, not unexpectedly, there is no cooperative behavior. The enzyme is insensitive to fructose-1,6-diphosphate. Patients with deficiency of red cell PK have normal PK2 and PK3.There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. Ab38237 recognizes the M1/M2 isoform of pyruvate kinase