Product Name :
JC-10 (ultra pure)
Sequence:
Purity:
≥95% (HPLC)
Molecular Weight:
~600
Solubility :
Soluble in DMSO.
Appearance:
Use/Stability :
Stable for at least one year after receipt when stored as recommended.
Description:
Mitochondria dye JC-10 is a derivative of JC-1 useful for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays.{{2481279-61-0} web|{2481279-61-0} Purity & Documentation|{2481279-61-0} Description|{2481279-61-0} manufacturer} JC-10 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. However, upon cell injury, as membrane potential decreases, JC-10 monomers are generated, resulting in a shift to green emission (525 nm).{{2560576-15-8} site|{2560576-15-8} Protocol|{2560576-15-8} References|{2560576-15-8} custom synthesis} The principal advantages of JC-10 relative to JC-1 include improved solubility in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss.PMID:30137783 JC-10 allows for both qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio, of mitochondrial membrane potential changes. Wavelength Maxima: Excitation 510nm, Emission 525nm
CAS :
Solubility:
Soluble in DMSO.
Formula:
Additional Information :
| Alternative Name Enhanced JC-1 | MW ~600 | Purity ≥95% (HPLC) | Solubility Soluble in DMSO. | Technical Info / Product Notes This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.