Limatization period of 15 days ahead of performing the experiments. All rats had been housed in metallic cages six in each and temperature maintained at 22+2 .STATISTICAL ANALYSISExperimental final results had been expressed as imply + SEM (n=6). Statistical evaluation was performed with one-way-ANOVA followed by Dunnetts t-test.RESULTSThe alcoholic extract of roots of Cissampelos pareira was subjected to qualitative phytochemical tests to identify the phytoconstituents and it revealed the presence of carbohydrates, alkaloids, sterols, phenolic compounds, tannins, flavonoids and resins. In acute toxicity study all of the animals had been survived even right after 14 days. This indicates that the extract was discovered to become safe up to the maximum dose level tested (2000 mg/kg). No big behavioural changes have been observed throughout this period of study. The results obtained with evaluation of diuretic activity of alcoholic extract of roots of Cissampelos pareira was shown in [Table/Fig1-3]. From the result it may be observed that alcoholic extract of roots of Cissampelos pareira has shown a significant diuretic activity by rising urinary output and improved excretion of sodium, potassium, chloride when compared to control. The impact of alcoholic extract of roots of Cissampelos pareira was found to be dose dependent, i.e., amongst the 3 doses studied, higher dose created extra impact. A comparison was produced with all the typical diuretic drug furosemide, the diuretic effect observed immediately after therapy with alcoholic extract of roots of Cissampelos pareira was located to be considerable in terms of urinary output, sodium, potassium, chloride concentrations. Determination of urinary electrolyte concentration revealed that alcoholic extract of roots of Cissampelos pareira was successful in growing urinary electrolyte concentrations for all of the three ions tested (Na+, K+, Cl-).EthicsThe experiment compiled together with the recommendations for animal experimentation of our laboratory and was authorized by the Institutional Animal Ethical Committee (IAEC). Drugs applied Furosemide 20 mg/ml (Sanofi Leptin Protein custom synthesis Aventis, Andheri East, Mumbai.)Acute toxicity studydetermination of ld50: The acute toxicity [14,15] of alcoholic extract of roots of Cissampelos pareira was determined by utilizing albino mice of either sex (16-20 g), maintained beneath regular husbandry circumstances. The animals have been fasted for three h before the experiment and also the extract was administered as single dose and observed for the mortality up to 48 h study period (quick term toxicity). Determined by the brief term toxicity profile, the following dose of your extract was determined as per OECD guidelines No.420. The maximum dose tested (2000 mg/kg) for LD50. In the LD50, doses like 1/20th, 1/10th and 1/5th had been selected and deemed as low, medium and high dose i.e., one hundred mg/kg, 200 mg/kg, 400 mg/kg respectively to carry out this study.Experimental DesignThe diuretic activity of alcoholic extract of roots of Cissampelos pareira in albino rats was studied by the Lipschitz Test [16-18]. Male Albino rats were divided into 5 GRO-beta/CXCL2 Protein Storage & Stability groups of 6 rats in every single. The group I serves as regular control received car (CMC 2 in regular saline 10 ml/kg b.wt), the group II received Furosemide (ten mg/kg, p.o) in car; other groups III, IV, V have been treated with low, medium, and higher doses of alcoholic extract of roots of Cissampelos pareira in car and right away immediately after the extract therapy all of the rats had been hydrated with saline (15 ml/kg) and placed inside the metabolic cages (two per ca.
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