Product Name :
SUPERFASLIGAND® Protein (soluble) (human), (recombinant)
Sequence:
Purity:
≥95% (SDS-PAGE)
Molecular Weight:
~32kDa (nonglycosylated), ~35kDa (glycosylated).
Solubility :
Appearance:
Use/Stability :
Stable for at least 6 months after receipt when stored at -20°C.
Description:
Fas ligand with improved stability providing significantly enhanced immune activation. Increased stability Enhanced immune activation compared to other recombinant ligands Mimics glycosylation of native human FasL SUPERFASLIGAND® (soluble) (human), (recombinant) features an N-terminus linker shown to improve stability and enhance immune activation compared to recombinant ligands alone. SDS-PAGE analysis: Lane 1: MW Marker, Lane 2: 1μg SuperFasL, stained with Imperial stain. Jurkat Cell Death: 50,000 Jurkat cells per well are incubated with the indicated concentration of SuperFasL for 3 hours. Cell death is determined by Cell-titer AQueous one-solution cell proliferation reagent. Formazan product is allowed to develop for 6 hours before the plate is read at 490nm. % Viability is determined in comparison to control well with no SuperFasL. SDS-PAGE analysis: Lane 1: MW Marker, Lane 2: 1μg SuperFasL, stained with Imperial stain. Jurkat Cell Death: 50,000 Jurkat cells per well are incubated with the indicated concentration of SuperFasL for 3 hours. Cell death is determined by Cell-titer AQueous one-solution cell proliferation reagent. Formazan product is allowed to develop for 6 hours before the plate is read at 490nm. % Viability is determined in comparison to control well with no SuperFasL.
CAS :
Solubility:
Formula:
Additional Information :
| Alternative Name FasL (oligomer), APO-1L (oligomer), CD95L (oligomer), CD178 (oligomer), TNFSF 6 (oligomer) | Application Notes ELISA: binds to Fas receptor at 1-100 ng/ml.1858276-04-6 Purity & Documentation | Biological Activity Kills Fas-sensitive cells.252917-06-9 In Vitro Note: Does not require enhancer.PMID:30676359 | Concentration 0.1mg/ml after reconstitution. | Endotoxin Content | Formulation Lyophilized. Contains PBS. | MW ~32kDa (nonglycosylated), ~35kDa (glycosylated). | Purity ≥95% (SDS-PAGE) | Reconstitution Reconstitute with 50µl sterile water. Further dilutions should be made with cell culture medium containing 5% fetal calf serum. | Source Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG®-tag. Glycosylation of rhs SUPERFASLIGAND® is similar to natural human FasL. | Specificity Binds to human, mouse and rat Fas (CD95; APO-1). | Technical Info / Product Notes Historical data has shown that SUPERFASLIGAND® kills Fas-sensitive cells at concentrations of >1ng/ml without the use of enhancer. The ED50 has been shown in previous data to be 1ng/ml (A20 cells).Note: Results using rhsSUPERFASLIGAND® may differ from those obtained with agonistic antibodies!FLAG is a registered trademark of Sigma-Aldrich Co. | UniProt ID P48023