Product Name :
Oligo(deoxyadenosine:deoxythymidine) (endotoxin-free) (synthetic)
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Use/Stability :
As indicated on product label or CoA when stored as recommended. Aqueous stock solution is stable for 1 day when stored at +4°C.
Description:
IFN activator Strong IFN inducer, dependent of IRF3.
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Additional Information :
| Alternative Name Oligo(dA:dT) | Couple Target IFN | Couple Type Activator | Endotoxin Content | Formulation Lyophilized. Sterile. | Quantity Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 0.{{187345-37-5} web|{187345-37-5} Protocol|{187345-37-5} In Vivo|{187345-37-5} supplier} 01-0.025µg/ml.{{166663-25-8} MedChemExpress|{166663-25-8} Purity & Documentation|{166663-25-8} In Vivo|{166663-25-8} custom synthesis} | Reconstitution For a 250µg/ml stock solution, dissolve the total vial content in 200µl endotoxin-free buffer or water.PMID:20301330 | Source Synthetic. | Technical Info / Product Notes For in vitro stimulation we recommend the following procedure:1. Bone marrow-derived mouse dendritic cells (BM mDC) are cultured 8×104/100μl in RPMI containing 10% FCS over night at 5% CO2 and 37°C.2. The medium is removed and replaced by 100μl OPTI MEM (Invitrogen) and subsequently the transfection mixture is added: 25μl OPTI MEM containing 0.025 to 0.25 Poly(dA:dT) + 25μl OPTI MEM containing 0.5μl Lipofectamine 2000 (Invitrogen) or alternative transfection agents.3. Over night stimulation at 5% CO2 and 37°C.4. Measurement of IFN-α/β in culture supernatants. | Unit of Measure (UM) µg