The MS spectra from the analytes and these of requirements there was a very good

The MS spectra from the analytes and these of requirements there was a very good match in the RT values and exact mz ratios of the [MH] and [MH] ions (Tables and) as well as on the MS spectra with the [MH] ions (Tables and).Frontiers in Plant Science www.frontiersin.orgNovember Volume ArticleSisTerraza et al.Coumarins in FeDeficient Arabidopsis PlantsFIGURE Chromatographic separation of a selection of phenolictype compounds produced in response to Fe deficiency by Arabidopsis thaliana roots.Typical fluorescence (at exc and em nm) and absorbance (at nm) chromatograms for root and growth media extracts from plants grown as described in Fourcroy et al. plants were pregrown for days in the presence of Fe (III)EDTA at pH then grown for days within a medium with (Fe) or Fe (III)EDTA (Fe) (the pH was not readjusted to with the final pH becoming c..in all pots).Chromatograms have been obtained applying Elution program .The encircled numbers above each peak correspond to the phenolic compounds listed in Table .RU, relative units, AU, absorbance units, and RT, retention time.4 a lot more compounds have been very first confirmed to become hexosidetype compounds from the RT, precise mz values and MS spectra in the [MH] ions.The RT values of these compounds (.min) have been close to those of recognized coumarin glucosides (.and .min for scopolin and fraxin, respectively), and lower than those of coumarin aglycones (.min for fraxetin, scopoletin, isofraxidin and fraxinol), phenylpropanoids (e.g .and .min for ferulic acid and sinapyl aldehyde), and glycoside and aglycone types of other phenolics (e.g min for flavonoids, stilbenes and lignans) (Supplementary Figures S and S).Hence, the RTs indicate that compounds are most likely to become polar (i.e hexoside) types of coumarins andor phenylpropanoids.Moreover, inside the MS(TOF) spectra, ions (positivenegative) at mz .and .for , , , and , respectively, were constant with the loss of a hexosyl moiety (.Da) from their corresponding [MH] [MH] ions (see mz values in Table).This was confirmed Elinogrel web making use of the low resolution MS spectra obtained with the ion trap major fragment ions (relative intensity at mz , , and inside the MS spectra of , respectively; Table) corresponded to the [MH] ions (mz , , and for , , , and , respectively) following a mass loss of Da.Precisely the same mass loss was also observed within the MS spectra of authenticated requirements with the coumarin glucosides scopolin and fraxin described above, with key ions at mz (scopolin) and (fraxin), corresponding together with the mz of their aglycones, scopoletin andfraxetin, respectively (Table).The rest of ions in the MS spectra of compounds , scopolin and fraxin showed significantly reduced relative intensities , indicating the hexosyl loss is favored.The aglycon moieties of compounds had been identified taking benefit of getting the dehexosylated ions inside the MS(TOF) spectra and also carrying out low resolution MS experiments around the ion trap.Very first, in the constructive and negative MS(TOF) spectra, the mz values for dehexosylated ions (see above) of , , , and have been assigned towards the elemental formulae C H O , C H O , C H O and C H O , respectively (with absolute errors ppm).Two of these elemental formulae, C H O and C H O , had been consistent with coniferyl and sinapyl aldehydes, involved in coumarin synthesis (Kai et al) (Table), whereas the other two, C H O and C H O , had been consistent with two coumarins already identified in the samples (compounds PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21541725 and , respectively) (Table).Ultimately, compounds were confirmed.