Dies used were caspase 3 and caspase 8 (Santa Cruz, Biotecnologies, USA). Immunohistochemistry was performed according to the manufacturer’s instructions.…
S. The protocol was approved by the Ethics Committee for Animal Experimentation (Service de la consommation et des affaires veterinaires,…
B in cellular transformation. Materials and methods Generation of Bub1 transgenic mice Bub1 transgenic mice were generated according to previously…
ion. DNA synthesis was plotted as percent of untreated control. In U266 cells there was a dose-dependent steady decrease reaching…
The amplitude of population spike responses to generate input?output curves (I/O curves). Paired-pulse facilitation was assessed by applying pairs of…
Ulatory T cells generation was due to increased apoptosis of CD4+ T cells. Surprisingly, cell death analysis using annexin V/podium…
Rtners or substrates of PRMT6. Some of the identified PRMT6’s partners support the reliability of our Y2H screening. hnRNP Q…
F the PI3K/Akt pathway, by overexpression of PTEN stimulates soluble Eng release from endothelial cells [35]. Given the link between…
O select the most appropriate reference gene, seven different housekeeping genes (glyceraldehyde-3-phosphate dehydrogenase (GAPDH), b2-microglobulin (B2M), beta-actin (ACTB), hypoxanthine phosphoribosyltransferase…
S were pooled and dialyzed against 1X phosphate buffered saline (PBS).24 h at 4uC, and dialyzed against 1X PBS thrice…