Name :
SG3/Secretogranin 3 Protein

Description :
Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs.

Species :
Cynomolgus

Uniprotkb :
HEK293

Tag :
C-His

Synonyms :
SCG3, Secretogranin-3, UNQ2502/PRO5990, SGIII, Secretogranin III

Construction :
Recombinant Cynomolgus SG3/Secretogranin 3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe20-Leu468.[Accession |I7G9U8]

Protein Purity :
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Molecular Weight :
The protein has a predicted MW of 52.14 kDa. Due to glycosylation, the protein migrates to 58-70 kDa based on Tris-Bis PAGE result.

Endotoxin :
Less than 1EU per μg by the LAL method.

Formulatione :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage :
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Research Background :
Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs.

References and Literature :
1. Li W, et al. Secretogranin III: a diabetic retinopathy-selective angiogenic factor. Cell Mol Life Sci. 2018 Feb;75(4):635-647. doi: 10.1007/s00018-017-2635-5. Epub 2017 Aug 30. PMID: 28856381; PMCID: PMC5771826.

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