Product Name :
Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein
EN
MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1
Description:
| Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein with the C-His-Avi Tag |Source: The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
Source:
The recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein, (amino acids Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide) fused with a His-Avi tag at the C-terminus, was produced in E.coli cells.
Molecular Weight :
The recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer protein with the His-Avi tag at the C-terminus has a predicted MW of 258 kDa. The protein migrates to 120-180 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on Tris-Bis PAGE result under Non reducing (N) condition.
Activity:
Immobilized recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody protein with the C-hFc tag with the EC50 of 1.9 ng/ml determined by ELISA.
Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.
Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. BP005236Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein with the C-His-Avi Tag SourceThe recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein, (amino acids Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and SLLMWITQC peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Shipping:
The recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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