mouse anti-MBP monoclonal antibody (F-6) 9051

Additiona Information:
9 5 2 inhostmouse|isotypeIgG2b|clonalitymonoclonal|concentration1 mg/mL|applicationsELISA, ICC/IF, WB|available sizes100 g|mouse anti-MBP monoclonal antibody (F-6) 9051antibodyDatabase link:P0AEX9Sequence – MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITKTested applicationsWB,ICC/IF,ELISARecommended dilutionsWB: 1:1000-3000ICC/IF: 1:500-2000For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.ImmunogenPurified recombinant MBP fusion protein expressed in?E. ColiSize and concentration100g and 1 mg/mLFormliquidStorage Instructions2-8C for short term, for longer term at -20C. Avoid freeze / thaw cycles.Storage bufferPBS, pH 7.2, 0.05% NaN3Purityaffinity purifiedClonalitymonoclonalIsotypeIgG1Compatible secondariesgoat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721Isotype controlMouse monocolonal IgG1 – Isotype Controltarget relevanceMBP can be used as a tag to aid in the expression and folding of fusion proteins. This antibody can be used to confirm expression and quantify the MBP tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, MBP tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.Click for more on: epitope tagsand MBPBiotechnologyMaltose-binding protein (MBP) has emerged as a highly effective and widely used fusion protein in biotechnology and recombinant protein expression. MBP is a stable, soluble, and well-folded protein that exhibits a strong affinity for maltose, making it an excellent tool for enhancing the expression and solubility of target proteins. In the context of fusion proteins, MBP is often genetically linked to a target protein of interest, allowing for simultaneous production of both proteins. The presence of MBP aids in proper folding and stability of the fused target protein, preventing aggregation and proteolytic degradation. Additionally, MBP can facilitate the purification of the fusion protein through affinity chromatography, where the fusion protein can be selectively eluted using maltose. This purification process greatly simplifies and expedites protein purification, ensuring high yields of correctly folded and biologically active target protein. Due to its versatile applications and benefits, MBP fusion proteins have become indispensable tools in structural biology, enzymology, and a wide range of biotechnological applications.|

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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