Product: Omadacycline (hydrochloride)
VAP-1/AOC3 Antibody (7-88) Summary
| Immunogen |
Vessels from mouse lymph nodes
|
| Isotype |
IgG2b
|
| Clonality |
Monoclonal
|
| Host |
Rat
|
| Gene |
AOC3
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
This antibody works in Flow Cytometry, Functional, Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation
|
Reactivity Notes
Mouse
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
0.2 um filtered solution in PBS and 0.1% BSA
|
| Preservative |
No Preservative
|
| Concentration |
0.1 mg/ml
|
| Purity |
Protein A or G purified
|
Notes
Positive Control: Mouse VAP-1-transfected CHO cells (ref. 2) Negative control: Mock-transfected CHO cells (Ref. 2)
Alternate Names for VAP-1/AOC3 Antibody (7-88)
- amine oxidase, copper containing 3 (vascular adhesion protein 1)
- AOC3
- Copper amine oxidase
- EC 1.4.3
- HPAO
- HPAOSSAO
- Semicarbazide-sensitive amine oxidase
- SSAO
- VAP1
- VAP-1
- VAP1EC 1.4.3.21
- VAP-1membrane primary amine oxidase
- Vascular adhesion protein 1
Background
The monoclonal antibody 7-88 recognizes mouse Vascular Adhesion Protein-1 (VAP-1) which is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N-terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 is expressed in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity. In white adipose tissue of obese and diabetic db-/- mice increased expression of VAP-1 has been observed suggesting that it contributes to the arthrosclerosis and vascular dysfunction observed in these diseases. Moreover, inhibition of VAP-1reduced the accumulation of myeloid cells into tumors and attenuates tumor growth. The monoclonal antibody 7-88 inhibits migration of granulocytes and monocytes in acute models of inflammation.