Product: Tyrosine kinase inhibitor
Semaphorin 5A Antibody Summary
| Immunogen |
Peptide with sequence DEATKKACYSKGKS corresponding to internal region according to NP_003957.1.
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| Specificity |
The immunizing peptide corresponds to a part of the extracellular domain.
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
SEMA5A
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| Purity |
Immunogen affinity purified
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| Innovators Reward |
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Preliminary experiments gave an approx. 90 kDa band in mouse brain lysates and approx. 25 kDa band in human brain (cerebellum), colon, heart and kidney lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121 kDa band according to NP_003957.1. The 90 kDa band and 25 kDa band were successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows membanous and vesicular staining in PCT cells.
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| Publications |
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Reactivity Notes
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Notes
Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at [email protected] for more details.
Alternate Names for Semaphorin 5A Antibody
- FLJ12815
- sema domain, seven thrombospondin repeats (type 1 and type 1-like)
- Sema F
- SEMA5A
- SEMAF
- Semaphorin 5A
- semaphorin F
- semaphorin-5A
- Semaphorin-F
- semF
- transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Background
Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met.