Rat IgG2a Isotype Control Summary
| Description |
This Rat IgG2A immunoglobulin is useful as an isotype control for Rat IgG2A antibody.
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| Specificity |
In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
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| Isotype |
IgG2a
|
| Host |
Rat
|
| Purity |
Protein G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20 degrees C or below. RAT IgG2a Lambda (?) isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: None.
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| Preservative |
0.01% Sodium Azide
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| Purity |
Protein G purified
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Alternate Names for Rat IgG2a Isotype Control
- IgG2A