Product: AI-10-50

Rat IgG1 Kappa Light Chain Isotype Control (KLH/G1-2-2) Summary

Description
This Rat IgG1 Kappa (KLH/G1-2-2) immunoglobulin is useful as an isotype control for Rat IgG1 Kappa antibody.
Immunogen
KLH
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat

Applications/Dilutions

Dilutions
  • Blocking/Neutralizing 50 – 200 molar excess
  • ELISA 1:100 – 1:2000
  • Flow Cytometry 1:10 – 1:1000
  • Fluorophore-linked immunosorbent assay 1:100 – 1:2000
  • Immunocytochemistry 1:10 – 1:500
Application Notes
Use the isotype control at the same concentration as the experimental antiobdy. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50 mM Tris/1 mM MgCl2/50% glycerol, pH 8.0.
Preservative
0.1% Sodium Azide
Concentration
Please contact technical services for concentration.

Background

RTK2071 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

PMID: 11242081