PRMT1 Antibody (PRMT1-171) Summary
| Immunogen |
Monoclonal Anti-PRMT1 is derived from the PRMT1-171 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with full length PRMT1.
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| Localization |
Nuclear
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| Specificity |
This reacts with PRMT1 and does not cross-react with PRMT2, 3, 4, and 5.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
PRMT1
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 2 ug/ml (determined by using a whole cell extract of the human kidney cell line 293T). Predicted molecular weight: 42 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
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Reactivity Notes
Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
10mM PBS (pH 7.4)
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| Preservative |
15mM Sodium Azide
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| Concentration |
2.0 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for PRMT1 Antibody (PRMT1-171)
- ANM1
- EC 2.1.1
- EC 2.1.1.-
- EC 2.1.1.125
- EC 2.1.1.77
- HCP1
- Histone-arginine N-methyltransferase PRMT1
- HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2
- HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae)
- HMT1
- HMT2
- HRMT1L2
- HRMT1L2HMT1 hnRNP methyltransferase-like 2
- Interferon receptor 1-bound protein 4
- IR1B4
- IR1B4protein arginine N-methyltransferase 1
- PRMT1
- protein arginine methyltransferase 1
Background
PRMTs are a family of proteins that either monomethylate or dimethylate the guanidino nitrogen atoms of arginine side chains. This common post-translational modification is implicated in protein trafficking, signal transduction, and transcriptional regulation. Most methyltransferases use the methyl donor, S-adenosyl-L-methionine (AdoMet), as a cofactor.