Product: MK-5172 (potassium salt)
NOP58 Antibody (34B12) Summary
| Immunogen |
A yeast nucleolar preparation
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
NOP58
|
| Purity |
Tissue culture supernatant
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
This NOP58 (34B12) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB a band can be seen at 57 kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW |
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Yeast.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
No buffer
|
| Preservative |
10mM Sodium Azide
|
| Purity |
Tissue culture supernatant
|
Alternate Names for NOP58 Antibody (34B12)
- HSPC120
- NOL5
- NOP5/NOP58
- NOP58 ribonucleoprotein homolog (yeast)
- NOP5nucleolar protein 58
- Nucleolar protein 5
- nucleolar protein NOP5/NOP58
Background
NOP58 (nucleolar protein 58) is a member of the conserved nop5 (Drosophila)/sik1/Nop56p (yeast) gene family that encodes components of snoRNP/small nucleolar ribonucleoproteins, which contains a snoRNA and a complex of proteins. NOP58 belongs to box C/D snoRNP, which is named for short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. snoRNP primarily catalyzes the cleavage as well as modification of rRNA during the biogenesis of ribosomes, nucleolar sizing, and snoRNA trafficking. In yeast, at least two members, Nop56 and Nop58, are present, and yeast cells deficient in either of the two members undergoes growth arrest because of defective protein translation. Furthermore, functional mutations of Nop56p/sik1 have been shown to cause reduced cell growth and proliferation. In mammalian cells, NOP58 has been found to increase rRNA synthesis and translational efficiency of the exogenous luciferase (Luc) gene, and its expression correlate with metastatic phenotypes of clinical melanomas.