NMDAR1 Antibody Summary
| Immunogen |
Peptide with sequence C-TQERVNNSNKKE corresponding to internal region according to NP_067544.1, NP_015566.1.
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| Marker |
Neuronal Marker
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| Specificity |
This antibody is expected to recognise all three reported isoforms (NP_000823.4; NP_067544.1; NP_015566.1).
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
GRIN1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Approx. 110 kDa band observed in rat brain lysates (calculated MW of 105 kDa band according to NP_015566.1). A minor band of unknown identity was also consistently observed at 23-24 kDa. This band was strong in mouse brain lysates and faint in human brain lysates. The band was successfully blocked by incubation with the immunizing peptide.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW |
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Positive Control |
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| Publications |
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Reactivity Notes
Predicted cross-reactivity based on sequence identity: Human, Mouse, Canine.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for NMDAR1 Antibody
- glutamate [NMDA] receptor subunit zeta-1
- glutamate receptor, ionotropic, N-methyl D-aspartate 1
- NMDA1
- NMDAR1glutamate [NMDA] receptor subunit zeta 1
- NMD-R1
- N-methyl-D-aspartate receptor channel, subunit zeta-1
- N-methyl-D-aspartate receptor subunit NR1
- NR1
Background
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. The gene consists of 21 exons and is alternatively spliced, producing transcript variants differing in the C-terminus. Although the sequence of exon 5 is identical in human and rat, the alternative exon 5 splicing in rat has yet to be demonstrated in human. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits.