1019)

By pyruvate-dehydrogenase inhibitor July 26, 2017

ICAM-3/CD50 Antibody (ICAM3/1019) Summary

Immunogen	Recombinant human ICAM3 protein
Localization	Cell surface
Specificity	Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.
Isotype	IgG2b Kappa
Clonality	Monoclonal
Host	Mouse
Gene	ICAM3
Purity	Protein A or G purified
Innovators Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovators Reward

Dilutions	Western Blot 0.5 – 1.0 ug/ml Flow Cytometry 0.5 – 1 ug/million cells in 0.1 ml Immunohistochemistry-Paraffin 0.5 – 1.0 ug/ml Immunofluorescence 0.5 – 1.0 ug/ml
Application Notes	Hu-chromosome location: 19p13.2 Molecular weight of antigen: 110-160kDa Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes.