Huntingtin Antibody (HDB4E10) Summary
| Immunogen |
Recombinant fragment, corresponding to amino acids 1844-2131 of Huntingtin.
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| Localization |
Cytoplasmic
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| Specificity |
This reacts with an epitope corresponding to the HDB region (amino acids 1844 – 2131) of the huntingtin protein.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
HTT
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Immunohistology on frozen (paraffin not tested). Western blotting detects a 350kD band on western blots but also detects smaller degradation products of huntingtin. This clone recognizes both denatured and native huntingtin in human brain.
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| Positive Control |
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Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for Huntingtin Antibody (HDB4E10)
- HD protein
- huntingtin (Huntington disease)
- huntingtin
- IT15HDHuntington disease protein
Background
The combined use of clones HDC8A4, HDB4E10 and HDA3E10 demonstrate that huntingtin is enriched in neuronal cells in the brain.