GnRH Antibody Summary
| Immunogen |
Synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH.
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| Specificity |
GnRH
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| Isotype |
IgG
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
GNRH1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
May be useful in IF and IHC-P. IF: Staining of GnRH in mouse embryo brain tissue results in staining of neurons in the rostral forebrain. IHC: 4% paraformaldehyde is recommended as a fixative.
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| Positive Control |
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Reactivity Notes
Mouse reactivity reported in scientific literatures (PMID: 21303944 and 25958044).
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
PBS with 1 mg/ml BSA
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| Preservative |
0.05% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for GnRH Antibody
- GNRH
- GnRH-associated peptide 1
- gonadotropin-releasing hormone 1 (leutinizing-releasing hormone)
- gonadotropin-releasing hormone 1 (luteinizing-releasing hormone)
- GRH
- LHRH
- LNRH
- luliberin I
- Progonadoliberin I
- progonadoliberin-1
- prolactin release-inhibiting factor
Background
Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle.Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.