Product: Acetylcholine (chloride)
ECSIT Antibody Summary
| Immunogen |
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human ECSIT protein.
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| Specificity |
This antibody is directed against human ECSIT protein. A BLAST analysis was used to suggest cross reactivity with ECSIT protein from human and mouse.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
ECSIT
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Expect a band approximately 45 kDa in size corresponding – ECSIT protein by western blotting in the appropriate cell lysate or extract.
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Reactivity Notes
Reactive in human, mouse
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| Preservative |
0.02% Sodium Azide
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| Purity |
Immunogen affinity purified
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Alternate Names for ECSIT Antibody
- ECSIT homolog (Drosophila)
- evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
- likely ortholog of mouse signaling intermediate in Toll pathway evolutionarilyconserved
- Protein SITPEC
- signaling intermediate in Toll pathway evolutionarily conserved ortholog
- SITPECsignaling intermediate in Toll pathway, evolutionarily conserved
Background
Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.