Chicken anti-Rat IgG (H+L) Secondary Antibody [DyLight 550] (Pre-absorbed)

Product: rac-Rotigotine (Hydrochloride)

Chicken anti-Rat IgG (H L) Secondary Antibody [DyLight 550] (Pre-absorbed) Summary

Immunogen
Purified Rat IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human, rabbit IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20 – 1:2000
  • Immunomicroscopy 1:20 – 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with sterilized deionized water. Centrifuge to remove any particulates.

Notes

Affinity purified antibody is > 95% based on SDS-PAGE. Chicken Ig fraction was prepared using eggs from healthy hens of US origin. DyLight 550 (Ex = 550 nm; Em = 576 nm). After reconstituted, prepare fresh working dilution daily.

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

PMID: 8709098