Product: Procarbazine (Hydrochloride)
Carbonic Anhydrase II/CA2 Antibody Summary
| Immunogen |
Carbonic anhydrase II purified from bovine erythrocytes
|
| Specificity |
Carbonic Anhydrase II
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
CA2
|
| Purity |
IgG purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
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|
Applications/Dilutions
| Dilutions |
- Western Blot 1:100-1:2000
- ELISA 1:100-1:2000
- Immunocytochemistry/Immunofluorescence 1:10-1:500
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Paraffin 5 ug/ml
- Immunoprecipitation 1:10-1:500
|
| Positive Control |
| Carbonic Anhydrase II/CA2 Lysate (NBL1-08615) |
|
|
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
|
| Preservative |
0.01% Sodium Azide
|
| Purity |
IgG purified
|
Alternate Names for Carbonic Anhydrase II/CA2 Antibody
Background
Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20
PMID: 10428375
Product: AMD-071 (hydrochloride)
Carbonic Anhydrase II/CA2 Antibody Summary
| Description |
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
|
| Immunogen |
Full length native Carbonic Anhydrase II (purified).
|
| Localization |
Cytoplasmic
|
| Specificity |
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
CA2
|
| Purity |
Ion exchange chromatography
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
- Western Blot 1:2000-1:10000
- ELISA 1:20000-1:100000
- Immunohistochemistry
|
| Application Notes |
This product has been assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and TMBE as a substrate for 30 minutes at room temperature.
|
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 9336012).
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
|
| Preservative |
0.01% Sodium Azide
|
| Purity |
Ion exchange chromatography
|
| Reconstitution Instructions |
Reconstitute with 0.1 ml deionized water to desired concentration.
|
Alternate Names for Carbonic Anhydrase II/CA2 Antibody
Background
Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20
PMID: 21440447