ARHGEF4 Antibody Summary
| Immunogen |
Peptide with sequence PWEEPAGEKPSCS corresponding to N-Terminus according to NP_056135.2, NP_127462.1.
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| Specificity |
This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1).
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
ARHGEF4
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Approx. 80 kDa band observed in human brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1 kDa band according to NP_056135.2 and 76.4 kDa band according to 127462.1).
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW |
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Positive Control |
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for ARHGEF4 Antibody
- APC-stimulated guanine nucleotide exchange factor 1
- Asef
- ASEF1
- ASEFAPC-stimulated guanine nucleotide exchange factor
- GEF4
- KIAA1112
- Rho guanine nucleotide exchange factor (GEF) 4
- rho guanine nucleotide exchange factor 4
- STM6
Background
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through Gucleotide exchange protein coupled receptors. Acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. This protein is similar to rat collybistin protein. Alternative splicing of the gene generates two transcript variants which encode different isoforms.